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Fig. 5. Effect of NO donors and NOS inhibitors on the inter-surge rate of rotation. Both 10 µmol l-1 SNAP (A) and 25 µmol l-1 SNP (B) caused a significant increase in the mean rate of slow tonic rotation. In contrast, 10 mmol l-1 L-NAME (C) and 100 µmol l-1 7-NI (D) caused a significant reduction in the mean rate of slow tonic rotation. Asterisks represent statistically significant differences compared to the control (artificial pondwater, APW) treatment. (A) P<0.001, N=32 embryos; (B) P<0.001, N=26 embryos; (C) P<0.001, N=14 embryos; (D) P<0.0001, N=22 embryos.