Fig. 7. Molecular confirmation that MYH13 is present in both orbital and global layers. (A) Schematic illustration of dissection of a 120 µm frozen section into two samples: the inner global layer (Glob.; light gray) and the outer (or intermediate) global layer plus the orbital surface layer (Orb.; dark gray). (B) The myosin isoforms present in EOM and in the two dissected samples (Orb. and Glob.) were analyzed by SDS-PAGE, which showed that MYH13 is present in the global as well as orbital layer.