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Fig. 3. Production of two antibodies specific for MYH13. Comparison of MYH13 with other MyHC sequences identified two highly divergent regions (EO-1, amino acids 1827-1938, and EO-2, amino acids 1210-1382). (A) Antibodies directed against those regions recognized only MYH13 on western blots containing extraocular muscle (EOM) and diaphragm muscle (Dia). (B) The migration positions of the myosin isoforms are shown on a duplicate gel stained for protein with Coomassie Blue G-250.