Fig. 4. Immunoreactivity (IR) associated with the ventral nerve cord (VNC) and ventral diaphragm (VD) of a day-3 adult P. unipuncta female. (A) Manse-AT-like immunoreactivity, dorsal view; (B) 5-HT-like immunoreactivity, lateral view. Whereas Manse-AT-like immunoreactive nerves run primarily on the dorsal surface of the collagen matrix (arrow), 5-HT-like immunoreactivity is seen primarily associated with nerves within the collagen matrix itself (double arrowheads). AM, alary muscles; CM, collagen matrix; TAG, terminal abdominal ganglia; VNC, ventral nerve cord. Scale bars, 100 µm.