Fig. 2. Verification of HSP70 by 2D-PAGE and immunoblot analysis in mouse liver. Liver tissues from control (A,C) and preconditioned (B,D) mice were the same as those described in Fig. 1. Gel electrophoresis and immunoblotting are described in Materials and methods. Commassie Blue-stained gels (A,B) and immunoblots for HSP70i (C,D) are shown. Larger arrowheads indicate the HSP70i locations; smaller arrowheads indicate other members of the HSP70 family. IEF, isoelectrofocusing; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Molecular mass of the protein spots (in kDa) and the pH value for the range of IEF gels are indicated to the right and bottom, respectively.