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Fig. 4. Hill plot for dialysed haemocyanin of Haliotis iris measured in the absence (circles) and in the presence (triangles) of 50 mmol l–1 Mg2+, in 0.1 mol l–1 Bis-Tris buffer at pH 7.7 and 15°C. KT and KR are estimates of O2 association equilibrium constants (mmHg–1) for the low-affinity (T, tense) and high-affinity (R, relaxed) forms, respectively. S, fractional oxygen saturation.