Fig. 2. The effects of dialysis against various ions on the O₂-affinity (logP₅₀) of Haliotis iris haemocyanin at 15°C, pH 7.0 (filled columns) and pH 7.7 (open columns). Labels above the columns refer to the concentrations (mmol l^–1) of Ca²⁺ (Ca), Mg²⁺ (Mg), D-lactate (D-l), and L-lactate (L-l). nh, native haemolymph; dHc, dialysed haemocyanin without added cofactor.