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Fig. 8. Photomicrographs showing whole-mount preparations of the toad lateral (A,B) and dorsal (C,D) aortae following processing for NADPH diaphorase histochemistry (A,C) and neural nitric oxide synthase (NOS) immunohistochemistry (B,D). Using both techniques, a plexus of neural (n)NOS-positive perivascular nerve fibres (arrows) was observed in the outer layers of the wall of both vessels. In addition, some larger nNOS-positive perivascular nerve bundles (A, arrowhead) were observed. Scale bars, 100µm.