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Fig. 2. (A) Tension recording showing the effect of acetylcholine (ACh) on the dorsal aorta. The aorta was exposed to endothelin-1 (ET-1; 10-8 mol l-1) until a maximal constriction was achieved and then ACh (10-5 mol l-1) was added. ACh caused a marked vasodilation. (B) Mean responses (% vasodilation) of pre-constricted lateral and dorsal aortae to acetylcholine (10-5 mol l-1; N=5).