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Fig. 3. The relationships between Cl- efflux (JoutCl) and external Cl- concentration ([Cl-]ext) in stingrays acclimated to either ion-poor Rio Negro water (soft water, triangles and solid line, N=7) or ion-rich hard water (circles and dashed line, N=6). Values are means ± 1 S.E.M. There was a specific significant difference between the two relationships (*P<=0.05) only around 300 µmol l-1. The thicker line shows the uptake kinetic relationship for JinCl from Fig. 1, and the arrows mark the intersection points of balance where JinCl=JoutCl.