Fig. 9. (A) Mesos layer (m) in post-shed skin (2 days) showing well-organized and continuous bilayers in intercellular domains (arrows). (B) High magnification ultrastructure of the layer in the same post-shed skin (RuO₄ post-fixation). Note decreased lamellar lipid inclusions (ll) as well as electron-lucent lipid inclusions (1) in the outer cell compared to the pre-shed skin in Fig. 8. Desmosomes (d) are clearly seen in this field. Scale bars, 0.1 µm. The inset shows light microscopic histochemistry of frozen section (10-12 µm) stained for neutral lipids. Note the improved staining in the stratum corneum compared with Fig. 8, inset. Scale bar for inset, 10 µm.