Fig. 5. Effects of Ruthenium Red. (A, B) Effects of Ruthenium Red on luminescence parameters assayed by stirring. Values are means ± S.D. (A) Effect of Ruthenium Red on relative luminescent response. Total luminescent capacity (TLC) was normalized to the mean of the control samples run on each date of testing (open squares). Mechanically sensitive luminescence was integrated over the first 6 s (1st 6 s MSL; filled circles) and over a total of 200 s (TMSL; filled diamonds) while stirring and normalized to TLC of each sample (N=3-10 for each treatment). (B) Effect of Ruthenium Red on maximum intensity of mechanically sensitive luminescence (Max I) normalized to TLC for each sample. (C) Effects of Ruthenium Red on motility and ecdysis. Cells not swimming in suspension (on bottom; filled circles) and empty theca (open circles) are expressed as proportions of pigmented cells (N=3-9). Values are means ± S.D. (D) Effect of Ruthenium Red on the response to developing Couette flow. Results are representative of five independent samples tested for each 0 µmol l^-1, 5 µmol l^-1 or 50 µmol l^-1 treatment.