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Fig. 4. Effects of 5 µmol l-1 BAPTA-AM treatment on other luminescent parameters. (A) Integrated luminescence stimulated by addition of BAPTA-AM (open bars) or control (filled bars) solutions, and mechanically sensitive luminescence (1st 6 s MSL) and total luminescent capacity (TLC) of the same samples 40-50 min later (N=4 for each treatment). Values are means ± S.D. Only 1st 6 s MSL was significantly different between treatments (t=-10.3, P<0.0001). (B) Response to 5 µmol l-1 ionomycin after 40-50 min of BAPTA-AM or control treatment. Total ionomycin-sensitive luminescence (TISL) integrated over 200 s and the first 12 s of integrated ionomycin-sensitive luminescence (1st 12 s ISL) were measured (N=4 for each treatment). Values are means ± S.D.