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Fig. 3. Effects of 5 µmol l-1 BAPTA-AM treatment. (A) Effect of stirring of samples tested at different times after treatment. BAPTA-AM treatment (open circles) decreased mechanically sensitive luminescence (integrated over 6 s stirring; 1st 6 s MSL) compared with control-treated samples (filled circles). The curve represents an exponential decay model fit to the data: y=3.0x108 e-0.0933t (r2=0.93), where y is the 6 s integrated MSL, and t is the treatment time in min. (B) Spontaneous glow emission of BAPTA-AM-treated (open circles) and control-treated (filled circles) samples. (C) Response of BAPTA-AM-treated and control-treated samples to developing Couette flow.