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Fig. 3. Relationship between PCO2 and pH of semen. Semen samples were equilibrated for 4 h at 10°C prior to pH analysis. 5-12 data points were collected from each of 6 males. (A) Curve represents the fourth order polynomial equation generated with all data. (B) Linear regression of pH as a function of the log of the PCO2. The line is given by the equation: pH=7.708-0.612 (logPCO2); r2=0.94 with 95% confidence limits for the slope being -0.653 to -0.571.