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Fig. 1. Western blot analysis of aquaporin 3 (AQP-3) in branchial tissues of silver eels maintained in freshwater (FW) or acclimated for 3 weeks to a seawater (SW) environment. The blot shows AQP-3 immunoreactivity in plasma membrane fractions isolated from the gills of SW-acclimated eels (lanes A-C) and FW eels (lanes D-F). Controls include the use of both pre-immune serum (FW eel membranes; PI, lane G) and peptide-negated antiserum (FW eel membranes; Pep., lane H), see Materials and methods. Individual lanes represent protein samples from separate membrane preparations from each experimental group. Gill tissue was removed from two fish for each membrane preparation. Size markers (right) are in kDa.