Fig. 5. Northern blots using total RNA (15 µg) showing the time course of changes in gill AQP3 mRNA expression during the seawater (SW) acclimation of yellow eels. Times indicate the period for either freshwater (FW) to SW acclimation or for FW to FW transfer of control eels. Time 0 fish were taken directly from the stock tank. The 7 kb band seen in the tissue blot (Fig. 2) was not visible on autoradiographs in this figure, although it was included in the quantification results (Fig. 6).