Fig. 7. Electron micrograph of a portion of a glial cell on the surface of a muscle fibre following TrnEAAT1 immunogold labelling. The gold particles are on the cytoplasmic side of the cell membrane where both the N- and C-terminal regions of the transporter are located. G, glial cell; RS, rete synapticum. Bar, 1 µm. (Inset) Cross-section (light micrograph) through a muscle fibre showing darkly stained glial cells (arrowheads) that insulate the axons (unstained) from the haemolymph (note similarity to electron micrograph). Tissue was probed with the glutamate transporter C-terminal antibody and visualized with secondary antibody-gold enhanced with silver (tissue stained with Toluidine Blue). MF, muscle fibre. Bar, 100 µm.