Fig. 4. Isolated larval skeletal muscle fibre stained with anti-C-terminal TrnEAAT1 antibody showing a complex pattern of neuronal innervation. A portion of trachea (T) is filled with air, with axon branches running over and under it. The semi-transparent nature of the preparation reveals that the branching continues around the entire surface of the muscle fibre. The fibre was stained with the alkaline phosphatase substrate Vector Blue. N, motor neuron; SB, segmental boundary. Bar, 500 µm.