Fig. 3. Whole-mount view of an abdominal segment of a caterpillar stained with the TrnEAAT1 C-terminal antibody. A branched network of nerves found on the surface of the skeletal muscle fibres labelled strongly with this antibody. The stained projections of these nerves do not appear to cross the segmental borders. The only other structures labelled by the antibody were the ganglia. FB, fat body; G, ganglion; NC, nerve cord; SB, segmental boundary; SM, skeletal muscle. The tissue was probed with antibody directed to the C-terminal region of the transporter protein. A matching pattern was produced by the N-terminal antibody in separate preparations (not shown). Bound antibodies were visualized with the alkaline phosphatase substrates BCIP/NBT. Bar, 1 mm.