Fig. 2. Immunoblots (western blots) of caterpillar and moth tissue samples probed with the N- and C-terminal-specific TrnEAAT1 antibodies. (A) Tissue extracts probed with the antibody to the C-terminal peptide; (B) extracts probed with antibody to the N-terminal peptide. Lanes: 1, moth head membrane fraction; 2, caterpillar skeletal muscle membrane fraction; 3, moth head water-soluble fraction; 4, caterpillar skeletal muscle water-soluble fraction. The transporter bands are detected in the membrane fractions as in Fig. 1, and the positions of marker proteins (kDa) are shown.