Fig. 1. Immunoblots (western blots) of crude protein extracts of High Five (H5) cells expressing TrnEAAT1 and non-expressing control cells probed with antibodies to the C-terminal (A) and N-terminal (B) peptide. The two antibodies produced similar patterns in the membrane fractions and failed to detect bands in the lanes containing the water-soluble fractions. Lanes: 1, H5-infected soluble fraction; 2, H5-infected membrane fraction; 3, H5 control water-soluble fraction; 4, H5 control membrane fraction. The positions of marker proteins (kDa) are shown.