Fig. 3. Homozygous rk⁴ flies show normal release of eclosion hormone at ecdysis and are responsive to injections of ecdysis triggering hormone (MasETH). (A,B) The eclosion hormone immunoreactivity in the ventral nervous system of rk⁴ flies before (A) and after (B) adult eclosion. The insets show the loss of large immunoreactive swellings after eclosion. (C) The latency to adult ecdysis when pharate adult rk⁴ homozygotes were injected with MasETH. The advance in the time to ecdysis is identical to that seen in normal flies. 5 nervous systems from each stage were assayed.