Fig. 6. Net change in currents (
I) elicited by 0.5
mmoll-1 free amino acids in 100 mmoll-1 (A)
Na+- or (B) K+-containing medium obtained at a holding
potential of -60 mV. Negative values represent blockade of net inward current,
as described in Materials and methods. Values were normalized to the CAATCH1
WT preferences (Feldman et al.,
2000; Quick and Stevens,
2001) for gating amino acids in Na+-containing (Pro) or
K+-containing (Thr) medium. KAAT1 WT values were also measured for
comparison using the same electrophysiological apparatus. Values are means +
S.E.M., N=3.