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Fig. 6. Net change in currents ({Delta}I) elicited by 0.5 mmoll-1 free amino acids in 100 mmoll-1 (A) Na+- or (B) K+-containing medium obtained at a holding potential of -60 mV. Negative values represent blockade of net inward current, as described in Materials and methods. Values were normalized to the CAATCH1 WT preferences (Feldman et al., 2000; Quick and Stevens, 2001) for gating amino acids in Na+-containing (Pro) or K+-containing (Thr) medium. KAAT1 WT values were also measured for comparison using the same electrophysiological apparatus. Values are means + S.E.M., N=3.