Fig. 2. Comprehensive bio-informatic analysis of 5' EST from A. gambiae cDNA library and data generation. A. gambiae cDNA libraries enriched for full coding clones were constructed. 5' EST sequences were systematically generated from the clones and analyzed for hits in nonredundant databases. Signal peptides were predicted by submission of the cluster sequences to the SignalP server, allowing the identification of putative secretory (S), housekeeping (H) or unknown (U) cDNAs. Putative functions were annotated according to database hits, when available.