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Fig. 6. Focal adhesion kinase (FAK) localisation in fast-twitch muscle. (A) Immunocytochemical analysis of fast-twitch normal extensor digitorum longus (N-EDL) muscle for FAK protein with serum A-17. Fibre typing was carried out by detecting the expression of slow (C) and fast (D) myosin isoforms and histochemical analysis of myofibrillar ATPase activity after preincubation at pH 10.5 (E). Arrows point to FAK-immunoreactivity at the sarcolemma. A negative control reaction with normal rabbit serum is also shown (B). The fibre types are indicated. Scale bar, 100 µm.