Fig. 3. Focal adhesion kinase (FAK) expression in normal slow- and fast-twitch muscle. (A) Soluble deoxycholate protein extract (10 µg) of slow-twitch normal soleus (N-SOL) and fast-twitch normal extensor digitorum longus (N-EDL) muscle was subjected to immunoblotting analysis using the C-FAK serum. The position of the FAK protein is indicated by an arrow. (B) A Ponceau-S-stained transfer membrane prior to detection showing that approximately equal amounts of protein were loaded. The sizes of molecular mass (MW) markers are indicated.