Fig. 2. Supercooling point (A), glycerol concentration (B), rate of water loss (C) and body water content (D) for Diplolepis spinosa, D. gracilis and D. radicum prepupae during winter acclimation at 5 °C. Data points that have, but do not share, letters are significantly different. Values are means ± S.E.M. (N=8). Some S.E.M. are within the size of the symbol.