Fig. 3. Expression of GSase mRNA in control- and NH₄Cl-exposed B. sinensis in different tissues. mRNA was detected using ribonuclease protection assays as described in the text. Each lane was loaded with sample that originally contained 10µg of total RNA; when yeast RNA was substituted for mRNA from B. sinensis as a control, no visible band was obtained (data not shown). Lanes P correspond to either the GSase or ß-actin probe loaded with yeast RNA without the RNase step.