Fig. 2. Comparison of rat Alrp expression with that of actin, mitochondrial subunit Vb of cytochrome oxidase (CoxVb) and mitochondrial cytochrome c (Cyt c). Samples (20 µg) of protein were separated in a non-reducing 4 % to 12 % gradient gel. The respective western blot was probed with antibodies specific for mammalian Alrp, actin, CoxVb and Cyt c as indicated. Proteins were analysed with (+) and without (-) reducing agent (20 mmol l^-1 dithiothreitol) in the sample buffer. The sample from muscle demonstrates dimer formation. The molecular masses (MW) of marker proteins are listed in kDa. Li, liver; K, kidney; H, heart; M, muscle, S, spleen; L, lung; B, brain; F, fatty tissue; LB, liver blood; vB, venous blood; aB, arterial blood; T, testis. DTT, dithiothreitol.