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Fig. 5. The effect of acclimation salinity and experimental osmolarity on the relative concentration of betaine in crab muscle. +, muscles exposed to 960mosmoll-1; x, muscles exposed to 720mosmoll-1; {circ}, muscles exposed to 640mosmoll-1. The horizontal bars are mean values for each acclimation salinity. The units on the y-axis are arbitrary. Two-way ANOVA indicated a significant effect of acclimation salinity but no effect of experimental osmolarity. N=3 for each treatment.