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Fig. 11 . Example of the effect of [Ca2+] on the oxygen affinity (P50 in mmHg) and cooperativity (n50) of the intact hemoglobin and its dodecamers at pH 7.0. Heme concentration, 0.32 mmol l-1. W-Hb, intact hemoglobin; D, dodecameric fraction. 1 mmHg=0.133 kPa. Open columns, [Ca2+]=0 mmol l-1; stippled columns, [Ca2+]=0.1 mmol l-1.