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Fig. 4. Effects of bumetanide on intracellular Cl- activity and on the effect of (A) Na+-free or (B) K+-free saline on intracellular Cl- activity in serotonin-stimulated tubule. Tubules were exposed to Na+-free or K+-free saline for the period indicated by the horizontal black bar. The horizontal grey bar indicates the time of exposure to 10-5 mol l-1 bumetanide.