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Fig. 2. (A) Representative HPLC profiles showing the principal products generated by Ciona intestinalis heart preparations. (B) Peak 1 has an ultraviolet profile of a conjugated triene chromophore with a wavelength of maximum absorbance ({lambda}max) of 269 nm, while material under peaks 2-5 has an ultraviolet profile of a conjugated diene with a {lambda}max of 235 nm. Peaks 2 and 4 co-elute with authentic 12- and 8-HEPE respectively. PGB2, prostaglandin B2 (internal standard); 8-HEPE, 8-hydroxyeicosapentaenoic acid; 12-HEPE, 12-hydroxyeicosapentaenoic acid; 8,15-diHEPE, 8,15-dihydroxyeicosapentaenoic acid.