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Fig. 3. Unidirectional Na+ fluxes of gills 6 from crabs acclimated to low and high salinity, perfused with 20 and 38 {per thousand} saline, respectively. Ouabain (5 mmol l–1) added to the perfusate significantly inhibited both Jin of crabs acclimated to low salinity (P<0.05, N=5) and Jout of crabs acclimated to high salinity (P<0.001, N=4).