Fig. 3. (A) 10.5 % SDS gel of rostral (R) and caudal (C) bass myofibrils, demonstrating the position of the troponin proteins relative to the myosin light chains. (B) Tropomyosin purification via hydroxyapatite column chromatography. Note that in the rostral column fraction, the faster migrating isoform, TnT-2, predominates while caudally, the slower migrating isoform, TnT-1, predominates. Abbreviations same as in Fig. 2.