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Fig. 1. cDNA and predicted amino acid sequence of the Na++K+-ATPase {alpha}-subunit from gills of the blue crab Callinectes sapidus. Stop codons in the 5' untranslated region (including one in-frame, underlined), the putative stop site and stop codons downstream from the stop site are indicated in red. A Kozak consensus sequence around the likely start codon (underlined) is shown in green. Transmembrane domains predicted by hydrophobicity analysis are shown in blue. The location of the degenerate primers NAK10F and NAK16R, employed in the initial amplification, are indicated by right- and left-pointing arrowheads respectively. The polyadenylation signal in the 3' untranslated region is indicated in violet. GenBank Accession Number AF327439