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Fig. 2. The effect of acclimation condition (control versus softwater) and treatment (Untreated, Sham, RU486-treated and spironolactone-treated, Spiron.) on morphometric measurements for rainbow trout gills. (A) Chloride cell density, (B) lamellar thickness, (C) interlamellar distance. Values are means ± 1 S.E.M. (N=4 for each group). *A significant difference between control and softwater-acclimated fish within the same treatment group; {dagger} a significant difference within an acclimation condition (control or softwater) from the untreated group (two-way ANOVA followed by Bonferroni’s post hoc multiple comparisons test, P<0.05).