
Fig. 8. PAF phosphorylcholine hydrolase activity in saliva of adult female Culex quinquefasciatus. Serotonin-induced saliva from six mosquitoes was incubated with 50 µl PAF (other conditions as for Fig. 2). At various time intervals, a sample was examined by mass spectrometry. (A) Time course of PAF-associated masses (524.3 and 543.3). (B) Mass spectrum obtained immediately after mixing the reaction mixture with the mosquito saliva. (C) Spectrum after incubation for 3 h. A control sample without saliva and incubated for 3 h had a spectrum identical to that in A.