Fig. 7. Molecular sieving chromatography of 25 pairs of homogenised salivary glands from Culex quinquefasciatus. (A) Ultraviolet absorbance of the eluate at 220 nm. Inset: Retention time of the enzymatic activity in relation to molecular mass (MW) standards. For more details, see Materials and methods. (B) PAF phosphorylcholine hydrolase activity as determined by disappearance of the masses associated with PAF. (C) PAF phosphorylcholine hydrolase activity as determined by appearance of the mass associated with the diacylglycerol product. (D) Action of the eluate fractions on PAF-induced platelet aggregation.