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Fig. 4. Mass spectrum of the product of the hydrolysis of PAF by Culex quinquefasciatus SGH matches the spectrum of 1-O-hexadecyl-2-acetyl-rac-glycerol. (A) Product of the incubation of PAF (100 µmol l-1 final concentration) with SGH from one pair of glands (25 µl final volume) for 2 h, after which 5 µl were diluted with acid 80 % methanol and analyzed by mass spectrometry, as described in Fig. 2. (B) Mass spectrum of authentic of 1-O-hexadecyl-2-acetyl-rac-glycerol.