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Fig. 4. Concentration-dependent in vitro ferrous (Fe2+; filled symbols) and ferric (Fe3+; open symbols) iron absorption in the three regions of the intestine of the European flounder, anterior ({blacksquare}, {square}), mid ({circ}, ) and posterior ({blacktriangledown}, {triangledown}). Values are means ± S.E.M. (N=3–6). At each concentration and in each region of the intestine, the rate of iron absorption in the presence of Fe2+ is significantly greater than that in the presence of Fe3+ (Student’s t-test, P<0.05).