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Fig. 5. Electron energy loss spectroscopy of calcium localisation in Polyorchis penicillatus swimming muscle. (A) Net calcium distribution image. This image is generated by computer subtraction of a background image taken at dE=330 eV from the image taken at dE=355 eV, which is above the edge of electron absorption specific for calcium (CaL2,3 edge 346 eV). The bright spots indicate the calcium signal. (B) Corresponding image taken using conventional TEM (zero energy loss). In both images the arrowhead indicates a desmosome and the arrow a subsarcolemmal vesicle. Scale bar, 0.5 µm. Mi, mitochondria; Me, mesoglea.