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Fig. 9. Expression pattern of Drosophila Na+/H+ exchangers (NHEs). Left-hand panels: RT-PCR using primers specific for DmNHE1 (top), DmNHE2 (middle) and DmNHE3 (lower panel). Predicted sizes for genomic (black arrow) and cDNA (white arrow) templates are shown. Templates: Genomic, genomic DNA; Whole fly, whole-fly cDNA; Head, head cDNA; Body, body cDNA; Tubule, Malpighian tubule cDNA; Larva, mixed larval cDNA; Pupa, mixed pupal cDNA; No template, no DNA control. Ladder is a 1 kb marker (Promega). Right-hand panels: the identity of the major bands labelled was verified by Southern hybridisation using DIG-labelled cDNA probes derived from an EST clone for DmNHE1 and DmNHE3 and from the previously sequenced tubule cDNA RT-PCR product for DmNHE2.