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Fig. 6. (A) Expression of Tmyostatin 1 (upper panel) and Tmyostatin 2 (lower panel) mRNA in myotomal muscle of male rainbow trout. Comparative RT-PCR of total RNA (1 µg) from fast-glycolytic (f) and slow-oxidative (s) muscles from five immature (lanes 1–10 from the left) and three spermiating (lanes 11–16 from the left) males. (B) Expression of Tmyostatin 1 (upper panel) and Tmyostatin 2 (lower panel) mRNA in myotomal muscle of female rainbow trout. Comparative RT-PCR of total RNA (1 µg) from fast-glycolytic (f) and slow-oxidative (s) muscles from five immature (lanes 1–10 from the left) and three spawning (lanes 11–16 from the left) females.