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Fig. 5. Expression of Tmyostatin 1 (A) and Tmyostatin 2 (B) mRNA in the myotomal muscle of developing rainbow trout embryos. Comparative RT-PCR of total RNA (1 µg) from the trunk of trout embryos at the eyed stage (lane 1), at hatching (lane 2) and in the myotomal musculature of a 5 g alevin (lane 3).