Fig. 2. The effect of a 2.5 h treatment with different concentrations of caffeine on release of symbiotic algae from Aiptasia pulchella. The control (C) anemones were held at 25°C, while the cold-shocked anemones (CS) were held at 12°C for 2.5 h. Cell release is expressed as a percentage of the total number of symbiotic dinoflagellates in the anemone [(released/released+retained)x100] released into the medium. Values are means + S.E.M., N=15 in each condition. *Significantly different from the control value, P0.01.