Fig. 5. Northern blot analysis of poly(A)⁺ RNA from epithelial tissue (p), eyestalk (e), gill (g), heart (h), hepatopancreas (hp) and muscle (m). The blot was hybridised to cDNA probe peJK2, and a final high stringency wash of 0.1x SSC/0.1 % SDS at 65 °C was included. The blot was exposed for 2 h (A), 4 h (B) and 14 h (C). The positions of the 28S and 18S rRNA bands are indicated on the right, and single-stranded HindIII-digested DNA size markers (kb) are indicated on the left. The positions of the bands are indicated by arrowheads; large arrowheads indicate the major eyestalk band (0.7 kb).