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Fig. 1. Representative immunoblot for V-H+-ATPase B-subunit in gill membrane enrichments from freshwater (lane 1), seawater-acclimated (lane 2) and seawater (lane 3) Atlantic stingrays. The positions of molecular mass markers (kDa) are shown. The antibody recognized a 60.5±1.08 kDa protein in gills (arrow). Note the reduction in band intensity from lanes 1 to 3.