Fig. 5. (A) Tissue-specific transcription of MLC2 and MLC3 genes. Northern blot analysis of total RNA (5µg) from the brain, gills, liver, intestine (smooth muscle), white muscle, red muscle, heart (cardiac muscle) and kidney. (B) RNA expression analysis of MLC2 and MLC3 genes during the development of Sparus aurata; northern blot analysis of total RNA (5µg) from larvae at different stage of development. kbp, kilobase-pairs; hpf, hours post-fertilization.